组织样品检测
- 胸腺：设计T细胞系差异性、成长发育；
- 淋疤结：T受损上皮体细胞、B受损上皮体细胞、DC受损上皮体细胞定量分析；
- 骨髓：B癌内部成长健康成长探讨方案，红癌内部成长健康成长探讨方案，造血干癌内部探讨方案；
- 肾组织安排：浅析炎性細胞侵润性；
- 肺团体：小鼠肺团体中抗体肉瘤人体细胞肉瘤人体细胞组建介绍，肉瘤或慢性炎症小鼠肺团体中抗体肉瘤人体细胞肉瘤人体细胞浸润性介绍；
- 皮肤吧：体细胞炎性侵润性了解；
- 脑细胞膜结构：剖析安排结构中炎性细胞膜浸泡情况发生。
调查应用案例一：胸腺T生殖细胞发育期
Population | Gating Step | ||||
Th Cells | Live single | mCD4+ | mCD8- | / | / |
Tc Cells | Live single | mCD4- | mCD8+ | / | / |
DP Cells | Live single | mCD4+ | mCD8+ | / | / |
DN Cells | Live single | mCD4- | mCD8- | / | / |
DN2 Cells | Live single | mCD4- | mCD8- | mCD44+ | mCD25+ |
DN3 Cells | Live single | mCD4- | mCD8- | mCD44- | mCD25+ |
DN1 Cells | Live single | mCD4- | mCD8- | mCD44+ | mCD25- |
DN4 Cells | Live single | mCD4- | mCD8- | mCD44- | mCD25- |
實驗事例二：肾脏免疫性组织细胞分折（12色方案设计）
Population | Gating Step | ||||
Total Leukocytes | Live single | mCD45+ | / | / | / |
Neutrophiles | Live single | mCD45+ | mCD11b+ | Ly6G+ | / |
Monocytes | Live single | Not Neutrophiles | mCD11b+ | Ly6C hi | / |
Eosinophils | Live single | Not monocytes | mCD11b+ | SSC-H hi | / |
Macrophage | Live single | Not eosinophils | mCD11b+ | F4/80+ | / |
DC Cells | Live single | Not eosinophils | mCD11b+ | mCD11c+ | / |
T Cells | Live single | Not eosinophils | mCD3+ | mCD335- | / |
B Cells | Live single | Not eosinophils | mCD19+ | / | / |
NK Cells | Live single | Not eosinophils | mCD3- | mCD335+ | / |
Th Cells | Live single | Not eosinophils | mCD3+ | mCD4+ | mCD8- |
Tc Cells | Live single | Not eosinophils | mCD3+ | mCD4- | mCD8+ |
研究成功案例三：脑聚集浸润性抗体神经元具体分析（10色规划）
Population | Gating step | ||||
Total Leukocytes | Live single | mCD45+ | / | / | / |
Neutrophiles | Live single | mCD45+ | mCD11b+ | Ly6G+ | / |
Monocytes | Live single | Not Neutrophiles | mCD11b+ | Ly6C hi | / |
Eosinophils | Live single | Not monocytes | mCD11b+ | SSC-H hi | / |
Macrophage | Live single | Not eosinophils | mCD11b+ | F4/80+ | / |
T cells | Live single | Not eosinophils | mCD3+ | mCD19- | / |
B cells | Live single | Not eosinophils | mCD19+ | mCD3- | / |
Th | Live single | Not eosinophils | mCD3+ | mCD4+ | mCD8- |
Tc | Live single | Not eosinophils | mCD3+ | mCD4- | mCD8+ |
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